Dual RNA-seq unveils noncoding RNA functions in host-pathogen interactions.
Westermann AJ, Förstner KU, Amman F, Barquist L, Chao Y, Schulte LN, Müller L, Reinhardt R, Stadler PF, Vogel J
Nature. Jan 2016. doi: 10.1038/nature16547
COMMENT: Dual RNA-seq is used to investigate Salmonella intracellular infection and replication after invasion. In addition of coding RNAs the authors also analyzes lncRNAs and sRNAs. The found expression of 145 known and 189 candidate Salmonella sRNAs and one of them , PinT, shoes to be very important in Salmonella functions in this intracellular state. In addition this sRNA is regulating in vitro very important human cell pathways. PinT Salmonella sRNA influences JAK–STAT signalling and elevates IL8 mRNA levels.
In summary, really interesting not only because of the important results regarding salmonella intracellular infection but also because the powerful methodology and approach