Comprehensive description of blood microbiome from healthy donors assessed by 16S targeted metagenomic sequencing.

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PubMed ID: 26865079

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Païssé S, Valle C, Servant F, Courtney M, Burcelin R, Amar J, Lelouvier B

Transfusion. May 2016. doi: 10.1111/trf.13477

COMMENT: This publication is a new ones confirming that the blood of healthy humans is not as sterile as previously supposed. The authors do a description of the microbiome in different fractions of the blood of 30 healthy individuals. They analyze 16S using quantitative PCR and NGS sequencing (V3-V4 hypervariable regions of the 16S rDNA with illumina MiSeq).

They analyze 3 different blood fractions:

  • buffy coat [BC]
  • red blood cells [RBCs]
  • plasma

The blood bacterial DNA was distributed in these percentages in the 3 blood fractions:

  • BC: 93.74%
  • RBCs: 6.23%
  • Plasma: 0.03%

They found differences between the bacterial profiles of the different blood fractions. This data are useful for the experimental design of microbiome studies using blood.

This publication includes useful data to get insight in the blood microbiome.


Raquel Tobes