Human colon mucosal biofilms from healthy or colon cancer hosts are carcinogenic.
Tomkovich S, Dejea CM, Winglee K, Drewes JL, Chung L, Housseau F, Pope JL, Gauthier J, Sun X, Mühlbauer M, Liu X, Fathi P, Anders RA, Besharati S, Perez-Chanona E, Yang Y, Ding H, Wu X, Wu S, White JR, Gharaibeh RZ, Fodor AA, Wang H, Pardoll DM, Jobin C, Sears CL
J Clin Invest. Mar 2019. doi: 10.1172/JCI124196124196
COMMENT: This study reveals that homogenates of biofilm-positive colon mucosa from patients with colorectal cancer and healthy subjects promote tumor development in colorectal cancer mouse models.
Here, we test the hypothesis that human colon biofilms comprise microbial communities that are carcinogenic in CRC mouse models.
biofilm-positive, but not biofilm-negative, human colon mucosal homogenates induced colon tumor formation in 3 mouse colon tumor models (...). Remarkably, biofilm-positive communities from healthy colonoscopy biopsies induced colon inflammation and tumors similarly to biofilm-positive tumor tissues. (...) 16S rRNA gene sequencing and RNA-Seq analyses identified compositional and functional microbiota differences between mice colonized with biofilm-positive and biofilm-negative communities.
These results suggest human colon mucosal biofilms, whether from tumor hosts or healthy individuals undergoing screening colonoscopy, are carcinogenic in murine models of CRC.